Impacts on Growth Rate of Wheat, Tomato and Tobacco by Using Different Antibiotics Against Agrobacterium Tumefaciens

Abstract

Plants has great nutritious value and extensively used as feast and famine for the development of agriculture sector of any economy. Researchers are trying to produce many genetically modified plants for improving their nutritional value. Many transformation techniques are used for this purpose, but agrobacterium mediated transformation system is very efficient and reliable method for production of transgenic plants. Agrobacterium strain EHA105 was found to be the most efficient with higher rate of transformation frequency, because it provides stable integration at lower copy number. Bacterial contamination is main problem when EHA105 is used for transformation. Antibiotics are only source available source for elimination of Agrobacterium. The main purpose of this study is to determine effect of different antibiotics on the growth of Agrobacterium tumefaciens and selected plant species. In this study, examined that effects of these antibiotics (CTX, LVX, AMX, AMC) on Agrobacterium as well as their effects on root and shoot germination of wheat, tobacco and tomato. It was observed that CTX > AMX > AMC >LVX rate was seen in vitro wheat germination, estimated that all four antibiotics gave good results at 250mg/L. But, AMX > CTX > AMC > LVX rate was observed in vitro tomato and tobacco germination, AMX gave maximum growth rate at all 250mg/L, 500mg/L ,1000mg/L concentrations in tomato, remaining CTX and AMC gave best results at 250mg/l in both plants. The O.D measurements and inhibitory activity presented that β-Lactam antibiotics including AMX, CTX and AMC can use for suppressing of Agrobacterium tumefaciens. But AMX gave maximum growth of inhibition. The findings of this study suggests that AMX is best antibiotic which has less effect of growth rates in plants and has maximum control on Agrobacterium tumefaciens.